Recombinant Oxyntomodulin

V. N. Stepanenko¹, R. S. Esipov^1 #, A. I. Gurevich¹, L. A. Chupova¹ and A. I. Miroshnikov¹

^#Phone: (495) 336-68-33; e-mail: esipov@mail.ibch.ru

¹Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russia

Received: May 27, 2006; in final form: August 28, 2006

Abstract.  An artificial gene encoding oxyntomodulin was obtained using chemical and enzymatic methods and cloned into Escherichia coli. A recombinant plasmid was constructed containing a hybrid oxyntomodulin gene and Ssp dnaB intein from Synechocystis sp. The expression of the resulting hybrid gene in E. coli, its properties, and the conditions of its autocatalytic cleavage to oxyntomodulin were studied.

Key words:  autocatalytic cleavage, intein, oxyntomodulin

Russian Journal of Bioorganic Chemistry 2007, 33 (2): 227-232