Development of a Rapid Method for the Detection of Prostate-Specific Antigen by Immunochromatography

I. A. Lubavina^1 #, A. A. Zinchenko¹, Yu. S. Lebedin² and S. V. Chukanov²

^#Phone: (495) 330-66-74; e-mail: lia@ibch.ru

¹ Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russia;
² Khema-Medica Co. Ltd., Moscow, Russia

Received: July 11, 2006;  in final form: September 13, 2006

Abstract.  A single-step qualitative rapid test for the determination of prostate-specific antigen (PSA) in samples of human blood serum by immunochromatography using a complex of colloidal gold with monoclonal antibodies to PSA as the detection agent was developed. The determination limit for PSA in serum blood samples is 10 ng/ml; the analysis time, 15–25 min; the sensitivity of the method, 100%; and its specificity, 92.5%.

Key words:  complex with colloidal gold; colloidal gold; immunochromatography; monoclonal antibodies to prostate-specific antigen; prostate-specific antigen; determination

Russian Journal of Bioorganic Chemistry 2007, 33(5): 511-515