Polycationic Catalysts for Phosphodiester Bond Cleavage on the Basis of 1,4-Diazabicyclo[2.2.2]octane

E. A. Burakova^1 #, N. A. Kovalev¹, I. L. Kuznetzova¹, M. A. Zenkova¹, V. V. Vlassov¹ and V. N. Sil’nikov¹

^#Phone: (383) 333-37-62; e-mail: kat7@ngs.ru

¹Novosibirsk Institute of Chemical Biology and Fundamental Medicine, Siberian Division, Russian Academy of Sciences, pr. Akademika Lavrent’eva 8, Novosibirsk, 630090, Russia

Received: June 22, 2006;  in final form: July 11, 2006

Abstract.  A number of tetracationic compounds capable of phosphodiester bond cleavage within a 21-membered ribooligonucleotide were designed and synthesized. The artificial ribonucleases represent two residues of quaternized 1,4-diazabicyclo[2.2.2]octane bearing alkyl substituents of various lengths and connected with a rigid linker. The efficiency of cleavage of phosphodiester bonds in an RNA target depends on the linker structure and the length of alkyl substituent.

Key words:  artificial ribonucleases; 1,4-diazabicyclo[2.2.2]octane; polycationic molecules

Russian Journal of Bioorganic Chemistry 2007, 33(5): 523-530