SYNTHESIS AND CHARACTERIZATION OF POLYSIALIC ACID/CARBOXYMETHYL CHITOSAN HYDROGEL WITH POTENTIAL FOR DRUG DELIVERY

2015 J. R. Wu#, X. B. Zhan, Z. Y. Zheng, and H. T. Zhang

#Phone +86 (510) 8591-8299; e-mail: kinowu@jiangnan.edu.cn

Key Laboratory of Carbohydrate Chemistry & Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122 China

Received September 10, 2014; in final form March 3, 2015

A novel hydrogel was prepared from polysialic acid (PSA) and carboxymethyl chitosan (CMCS) using glutaraldehyde as the cross-linking agent. The resulting PSACMCS hydrogel exhibited pH sensitivity, in which the swelling ratio under acidic conditions was higher than those under neutral or alkaline conditions. The swelling ratio of PSACMCS hydrogel at equilibrium depended on the medium pH, the cross-linking agent concentration, and the ratio of PSA to CMCS (w/w). Bovine serum albumin (BSA) and 5-fluorouracil (5-FU) were used as model drugs to prepare hydrogel delivery systems. The loading efficiencies of the hydrogel for BSA and 5-FU were 26.25 and 36.74%, respectively. Release behaviors of BSA and 5-FU were influenced by the pH. MTT assays confirmed that PSACMCS hydrogel has no cytotoxicity toward the NIH-3T3 cell line; in fact, the 100% aqueous extract of the PSACMCS hydrogel enhanced cell growth. These results suggest that PSACMCS hydrogel may be a promising pH-sensitive delivery system, especially for hydrophobic chemicals.

Keywords: polysialic acid, carboxymethyl chitosan, hydrogel, drug delivery.

, 2015, 41, 5, . 627632