GREEN SYNTHESIS OF ISOAMYL ACETATE VIA SILICA IMMOBILIZED NOVEL THERMOPHILIC LIPASE FROM BACILLUS AERIUS

© 2016 Sunil Kumar Narwal*, Nitin Kumar Saun*, Priyanka Dogra**, and Reena Gupta*^{, #}

^#E-mail: reenagupta_2001@yahoo.com

*Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla, 171005 India;
**Department of Chemistry, Himachal Pradesh University, Summer Hill, Shimla, 171005 India

Received January 15, 2015; in final form, September 1, 2015

Isoamyl acetate, a pear or banana flavor, is widely used in food, beverage, cosmetic, and pharmaceutical industries. In the present work, lipase from Bacillus aerius was immobilized on silica gel matrix in the presence of a cross-linking agent, glutaraldehyde, and its efficiency in synthesizing isoamyl acetate using esterification reaction was studied. The esterification of acetic acid and isoamyl alcohol by silica-bound lipase was studied as a function of time and temperatures. The incubation time of 10 h, temperature of 55°C, substrate molar ratio 1 : 1, and the amount of lipase as 1% were found to be optimal for the esterification reaction. The bound lipase catalyzed the esterification of acetic acid by isoamyl alcohol with the yield of about 68% under the optimized reaction conditions. The product was identified as isoamyl acetate using gas–liquid chromatography, nuclear magnetic resonance, and Fourier transform IR spectroscopy analysis by the presence of an ester group at the wavenumber of 1720.5 cm^–1.

Keywords: lipase, immobilization, esterification, isoamyl acetate.

Биоорг. химия 2016, 42 (1): 79-83