DEVELOPMENT, OPTIMIZATION, AND BIOVALIDATION OF 99mTc–INSULIN COMPLEX
© 2016 Muhammad Usman Akbar*, **, #, Tanveer Hussain Bokhari*, Samina Roohi***, Khalid Mahmood Zia**, Mohammad Zuber**, Nadia Parveen****, and Narmeen Ali*
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*Department of Chemistry, Government College University, Faisalabad, 38000 Pakistan; **Department of Applied Chemistry and Biochemistry, Government College University, Faisalabad, 38000 Pakistan; ***Isotope Production Division, Pakistan Institute of Nuclear Science and Technology, P.O. Nilore, Islamabad, Pakistan; ****Pakistan School Muscat Sultanate of Oman
Received December 7, 2015; in final form, April 19, 2016
Human biosynthetic insulin is a polypeptide hormone that plays an important and essential role in control of the level of carbohydrate, protein, and fat metabolism in the blood. Human pancreatic insulin was labeled with 99mTc to form a new radiopharmaceutical with a labeling yield of 99 ± 1% under optimum conditions: 0.1 mL insulin, pH 7, 25 μg stannous chloride, 1 mL (19 mCi) of pertechnetate, room temperature, and 10 min reaction time. The 99mTc–insulin complex was examined using paper chromatography, ITLC, electrophoresis, and HPLC. In addition, in vitro and in vivo study of 99mTc–insulin complex was performed at different
Keywords: insulin, technetium-99m, labeling, optimization, HPLC, electrophoresis, biodistribution.
Áčîîđă. őčěč˙ 2016, 42 (5): 546-551