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NUCLEIC ACID HYBRIDIZATION ANALYSIS USING STAINED LATEX PARTICLES
T. I. VENER, M. F. TUHCHINSKY, V. D. KNORRE, A. N. GENERALOVA, Vu. V. LUKIN, S. N. SCHERBO, S. I. TURKIN, V. P. ZUBOV
M. M. Shemyakin Institute of Bioorganic Chemistry, Academy of Sciences of the USSR, Moscow
Abstract: Method of the latex hybridization analysis has been developed: after hybridization of NA-target with biotinylated probe visualization of hybrids was carried out using latex particles, containing fluorescent dye pyronin G and coated with streptavidin. Due to in-capsulation of the fluorescent dye in polymer particles sensitivity of the analysis was increased by several orders of magnitude in comparison with methods, using fluores-cently labelled probes. Possessing a number of advantages, the method yields to none of any other methods of NA hybridization analysis in sensitivity.
Russian Journal of Bioorganic Chemistry 1990, 16 (3):424-426