Photomodification of Nucleic Acids by N-(2-Oxyethyl ) phenazine Derivatives of Oligonucleotides

V. F. Zarytova, I. V. Kutyavin, T. V. Maltseva, S. V. Mamaev, V. P. Maltsev

Novosibirsk Institute of Bioorganic Chemistry , Siberian Division of the Academy of Sciences of the USSR: *Novosibirsk Institute of Chemical Kinetics and Combustion, Siberian Division of the Academy of the USSR

Abstract: Photomodification of a 302-membered single-stranded DNA fragment by 5'-mono-and 3',5'-di-N-(2-oxyethyl)phenazine (Phn) derivatives of oligonucleotides has been investigated. Under strong laser irradiation (λ 532 nm; power density 2,5 GV/cm2, irradiation dose 30 J) the DNA fragment in the presence of Phn-reagents was significantly destructed (up to 7095%). The level of complementary addressed modification (24 51%) is a direct function of the length of oligonucleotide address of the photoreagent and the amount of Phn residues, stabilizing the complementary complex. The character of the nonaddressed modification is close to the statistic one, although for a number of photoreagents a rather efficient nonspecific modification of 5'-terminal sequence of target DNA has been detected. Of interest also is an unusually broad positional direction of the DNA fragment photomodification in the area of perfect complementary coupling: of 5'-Phn-reagents.

Russian Journal of Bioorganic Chemistry 1990, 16 (5):595-602

Full Text (PDF, in Russian)