Alkylation of nucleic acid components by ethylenimide derivatives. I. Alkylation of bases

T. P. Voloshchuk, Yu. V. Patskovsky, A. I. Potopalsky

Institute of Molecular Biology and Genetics, Academy of Sciences of the UkrSSR, Kiev

Abstract: In the course of investigating the reaction conditions of the nucleic acid components alcylation, the interaction of thioTEPA (N,N',N"-triethylenethiophosphoamide) with hydrochloric and perchloric acids was studied, perchloric acid increasing the alkylation products yield. HPLC and UV spectroscopy were used to isolate and identify products of nucleic bases alkylation by ethylenimine and its derivatives (thioTEPA and mono-aziridinediethylphosphate). It is shown that under neutral conditions phosphoamino-ethylation takes place, whereas under slightly acidic conditions products of aminoethyl-ation are formed.

Russian Journal of Bioorganic Chemistry 1990, 16 (7):981-990

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