Synthesis, cloning and expression of artificial genes coding for antigenic determinants of the foot-and-mouth disease virus strain A₂₂

V. G. Korobko, E. F. Boldyreva, O. V. Nekrasova, A. Mikulskis, S. A. Filippov, V. N. Dobrynin

M. M. Shemyakin Institute of Bioorganic Chemistry, Academy of Sciences of the USSR, Moscow

Abstract: Chemical-enzymatic synthesis and cloning in Escherichia coli of double-stranded DNAs, coding for simple and complex antigenic determinants of foot-and-mouth disease virus (FMDV) strain A₂₂, have been carried out. The simple antigenic determinants are a part of the viral coat protein VP1 (amino acid sequence 131–152 or 131–160) whereas the complex antigenic determinants comprise additionally the amino acid sequence 200–213 of VP1 linked to N-terminus of simple antigenic determinants through a tetrapeptide spacer Pro-Pro-Ser-Pro. Recombinant DNAs containing genes for antigenic determinants of FMDV fused with C-terminus of gene for human tumor necrosis factor (hrTNF) have been constructed. Expression of the hybrid genes and properties of the proteins coded were studied. All recombinant proteins were shown to interact specifically with polyclonal antibodies both against hrTNF and FMDV strain A₂₂. The recombinant proteins produced by bacteria are perspective for study as a vaccine against FMDV.

Russian Journal of Bioorganic Chemistry 1991, 17 (4):461-469

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