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Use of affinity sorbents for study of phospholipase A2 interaction with substrates
N. G. Evstratova, G. A. Serebrennikova
M. V. Lomonosov Institute of Fine Chemical Technology, Moscow
Abstract: Preparation of biospecific sorbents of the polysaccharide type involving different phospholipid ligands is described. By means of affinity chromatography, the formation of the phospholipase A2 – phospholipid complex was quantitatively characterized. Two types of the complexes are formed on this sorbents, with lipid – enzyme ratios (40–154): 1 and (5–7): 1 depending on the lipid concentration. Choice of biospecific sorbents for affinity chromatography of phospholipase A2 is discussed.
Russian Journal of Bioorganic Chemistry 1991, 17 (4):567-571