Synthesis of tripeptide glycyl-L-leucyl-L-phenylalanin.6 and its analogues

O. V. Esipova, S. V. Eremin, E. N. Zvonkova

M. V. Lomonosov Institute of Fine Chemical Technology, Moscow

Abstract: Peptide Gly-L-Leu-L-Phe and its derivatives were synthesized by the C-end elongation utilizing DCC/HOBT technique and by enzymatic route with the help of papain using esters of N-benzyloxycarbonyl-glycine and -L-leucine as acyl donors have been suggested. The chemical, similarly to the enzymatic, synthesis was not accompanied by racemization. Conditions for HPLC separation and preparative isolation of the enzymatic reaction products were developed.

Russian Journal of Bioorganic Chemistry 1991, 17 (8):1077-1085

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