Russian Journal of Bioorganic Chemistry, Vol. 21, No. 4, 1995, p. 209–213

A new Differentiation Factor from HL-60 Cell Culture Treated with Retinoic Acid: Isolation and Sequencing

I. A. Kostanyan, M. V. Astapova, E. V. Starovoitova, S. M. Dranitsyna, and V. M. Lipkin

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, V-437, GSP-7, Moscow , 117871 Russia

Abstract: A new 8.2-kDa protein factor was isolated from cell culture of a promyelocyte leukemic HL-60 cell line that exhibited a differentiation-inducing effect upon the initial line. The primary structure of this factor was determined by sequencing the protein (N-terminal region) and the corresponding cDNA. A molecule of the differentiation factor secreted by HL-60 cells consisted of 54 amino acid residues and was glycosilated.

Key words: HL-60, differentiation factor, retinoic acid.