Russian Journal of Bioorganic Chemistry, Vol. 21, No. 7, 1995, p. 429–435

Automatic SynChrom System for Solid Phase Peptide Synthesis and Liquid Column Chromatography. Part I. Principles of Design and Structural Constituents

M.B. Baru , V.V.Cherskii ^* , A.V. Danilov, S.A. Moshnikov, and L.G. Mustaeva

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino Branch, Moscow , Pushchino, oblast’, 142292 Russia , *SynChro NPO, Pushchino, Moscow oblast’, 142292 Russia

Abstract: An automatic modular SynChrom system was designed for solid phase synthesis and chromatographic purification of peptides. Structural constituents and organization and operation of the system in solid phase peptide synthesis and liquid column chromatography modes are described. Swellographic monitoring of the course of synthesis was used. Hydraulic diagrams, operation algorithm and software are presented.

Key words: solid phase peptide synthesis, automatic synthesizer, liquid column chromatography, continuous-flow reactor with variable volume, swellographic monitoring

Russian Journal of Bioorganic Chemistry, Vol. 21, No. 7, 1995, p. 436–445

Automatic SynChrom System for Solid Phase Peptide Synthesis and Liquid Column Chromatography. Part II. Application to Solid Phase Peptide Synthesis and Liquid Column Chromatography

M.B. Baru, V.V.Cherskii ^* , A.V. Danilov, S.A. Moshnikov, and L.G. Mustaeva

Abstract: Automatic solid phase peptide synthesis using the SynChrom system is described. Problems of swellographic monitoring are discussed. Combined monitoring (swellographic, spectrophotometric, and manometric) of all steps of the synthetic cycle are suggested. Potential application of the system to liquid column chromatography werendemostrated.

Key words: solid phase peptide synthesis, automatic synthesizer, liquid column chromatography, continuous-flow reactor with variable volume, swellographic, spectrophotometric and manometric monitoring