Russian Journal of Bioorganic Chemistry, Vol. 22, No. 6, 1996, p. 368
Cloning of the ha-SDGF Gene cDNA from a Highly Metastatic Golden Hamster Cell Line by Subtractive Hybridization
N. G. Gurskaya*, D. A. Shagin*, K. A. Luk’yanov*, L. L. Vagner*, M. S. Shtutman**, E. A. Musatkina**, E. V. Moinova**, A. G. Tatosyan**, S. A. Luk’yanov*, 1 and E. D. Sverdlov*
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow , GSP-7, 117871 Russia
** Blokhin Cancer Research Center, Institute of Cancerogenesis, Russian Academy of Medical Sciences, Kashirskoe sh. 24, Moscow, 115478 Russia
Abstract: Using subtractive hybridization, a cDNA library containing over 50% of clones specific for a highly metastatic cell line was obtained from two hamster embryo fibroblast lines with different metastatic potentials. Most of the clones (83%) contained new sequences. One clone contained the ha-SDGF gene cDNA homolo-gous to SDGF cDNA from rodents. The level of ha-SDGF mRNA expression was considerably higher in the highly metastatic cell line.
Key words: subtractive hybridization, cell line, metastatic potential, cloning, polymerase chain reaction, ham-ster, growth factor