Russian Journal of Bioorganic Chemistry, Vol. 23, No. 7, 1997, p. 518

Interaction of Derivatives of Short Oligonucleotides with Nucleic Acids. II. ¹ A Tandem of Short Oligonucleotides as a Highly Sensitive System for Identification of Single-Base Substitutions in Target DNAs

D. V. Pyshnyi, M. A. Podyminogin, I. A. Pyshnaya, S. G. Lokhov, E. M. Ivanova, ² and V. F. Zarytova

Novosibirsk Institute of Bioorganic Chemistry, Siberian Division, Russian Academy of Sciences, pr. Akademika Lavrent’eva 8, Novosibirsk, 630090 Russia

Abstract: A new approach for modification of target DNAs with tandems of derivatives of short oligonucleotides was suggested that allows highly selective modification of perfect duplexes only. At physiological temperatures, the efficiency of DNA modification by a dodecanucleotide alkylating agent was demonstrated to be the same for both perfect and mismatch-containing duplexes, whereas the tetranucleotide reagent in the presence of two flanking effectors alkylated with high selectivity the target DNA in the perfect duplex only.

Russian Journal of Bioorganic Chemistry, Vol. 23, No. 8, 1997, p. 585

Interaction of Short Oligonucleotide Derivatives with Nucleic Acids: III. ¹ Photomodification of DNA Targets Using Tandems of Short Oligonucleotide Derivatives

D. R. Tabatadze, L. V. Tret’yakova, A. S. Levina, D. V. Pyshnyi, E. I. Ivanova, ² and V. F. Zarytova

Novosibirsk Institute of Bioorganic Chemistry, Siberian Division, Russian Academy of Sciences, pr. Akademika Lavrent’eva 8, Novosibirsk, 630090 Russia

Abstract: High efficiency was demonstrated for the photomodification of a DNA target by a 5'-p-azidotetrafluorobenzoyl reagent based on a tetranucleotide and its 3'-phosphoestrone ester in the presence of a pair of flanking effectors. These effectors are oligonucleotide derivatives with N-(2-hydroxyethyl)phenazinium groups or those connected to cholesterol residues at the terminal phosphates.