Russian Journal of Bioorganic Chemistry, Vol. 26, No. 8, 2000, đ . 512

The Three-dimensional Structure of the Antigen-binding Fragment of a Monoclonal Antibody to Human Interleukin-2 in Two Crystal Forms at 2.2 and 2.9 Resolution

A. V. Fokin*, P. V. Afonin*, I. Yu. Mikhailova*, I. N. Tsygannik *, T. Yu. Mareeva*, V. A. Nesmeyanov*, W. Pangborn**, N. Lee**, W. Duax**, E. Ciszak***, and V. Z. Pletnev* 1

*Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, GSP-7 Moscow , 117871 Russia , ** Hauptman–Woodward Medical Research Center , Buffalo , NY , 14203 United States ***NASA, Marshal Space Flight Center , Hantsweel , AL , United States

Abstract: The three-dimensional structure of the antigen-binding fragment of a monoclonal antibody to human interleukin-2 was determined in two crystal forms by the X-ray method of molecular replacement at 2.2 and 2.9 Å resolutions. The spatial structure of the protein and the stereochemistry of its antigen-binding site were analyzed.

Key words: monoclonal antibody, antigen-binding fragment, interleukin-2, X-ray analysis