Russian Journal of Bioorganic Chemistry, Vol. 31, No. 6, 2005, đ . 521

Properties and Functions of a New Nucleolar Protein, Surf-6, in 3T3 Mouse Cells

V. V. Gurchenkov 1, 2 , M . A. Polzikov 1 , Ch. Magoulas 3 , L. G. Romanova 1 , and O. V. Zatsepina 1, *

1 Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry , Russian Academy of Sciences , ul. Miklukho-Maklaya 16/10, Moscow, 117997 Russia, 2 Faculty of Bioengineering and Bioinformatics, Moscow State University, Vorob’evy gory, Moscow, 119899 Russia, 3 Queen Mary University, Mile End Road, London, E1 4NS, UK

Abstract: The localization of the specific protein Surf-6 from nucleoli of eukaryotic cells in mitosis and its sensitivity to the treatment of cells with RNase A and DNase I in situ were studied. It was shown that, in interphase nucleoli of 3T3 mouse cells, Surf-6 is probably associated with RNA and practically is not associated with DNA. In mitosis, Surf-6 appears in forming nucleoli after the known RNA-binding proteins fibrillarin and B23/nucleofozmin, which are involved in the early and late stages of the assembly of ribosomal particles, respectively. These observations and the regularities of migration of early and late proteins of ribosome assembly to nucleoli in the telophase of mitosis led us to the presumption that Surf-6 is involved in the terminal stages of the assembly of ribosomal particles in murine cells. An immunoblot analysis of the Surf-6 content in synchronized 3T3 cells showed for the first time that Surf-6 is present at all stages of the cell cycle but its content markedly decreases when cells enter the G0 period. Conversely, the activation of cells for proliferation is accompanied by an increase in the Surf-6 content. These observations allow one to regard Surf-6 as a marker of the cell proliferative state and suggest its implication in the regulation of the cell cycle.

Key words: B23/nucleofozmin, cell cycle, fibrillarin, mitosis, nucleolus, protein Surf-6