Russian Journal of Bioorganic Chemistry, Vol. 31, No. 2, 2005, đ . 178

The NOLA2 and RPS3A Genes As Highly Informative Markers of Human Squamous Cell Carcinoma of Lung

D. K. Slizhikova, T. V. Vinogradova, 1 and E. D. Sverdlov

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences,ul. Miklukho-Maklaya 16/10, Moscow , 117997 Russia

Abstract: cDNA libraries enriched with sequences that are differentially transcribed in normal and tumor tissues were prepared using the subtractive hybridization of mixtures of cDNAs from ten patients with squamous cell carcinoma (lung cancer) and the corresponding mixtures of cDNAs from normal tissues of the same patients. An analysis of the libraries revealed two genes, NOLA2 and RPS3A, whose expression in patients with squamous cell carcinoma increased by 70%. A high frequency of enhanced expression of these genes in the cancer makes them highly informative markers of squamous cell carcinoma, which, together with other markers, can be used for reliable diagnosis of the disease.

Key words: gene expression, human squamous cell carcinoma, lung cancer